Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 53, Issue 1, Pages 77-86Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(02)00226-9
Keywords
bacteria; enumeration; counting
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A simple method for nonspecific determination of bacteria concentrations in a variety of liquid samples was developed. The assay was based on the time required for a sample grown in liquid media to reach a threshold turbidity. Samples were combined with media in a covered 96-well microwell plate and the turbidity was monitored in real time as the bacteria grew in a temperature-control led plate reader. A significant problem with growth in microwells was condensation on the cover, which prevented accurate turbidity measurement. This problem was overcome by coating the cover with a small amount of surface-active agent. Salmonella and E. coli concentrations could be determined with a relative error of approximately 20% at levels from 10 to 10(6) cells/ml (eight replicates). An assay of 10 samples with standards required 10 min to set up and 20 min for data processing using a computer spreadsheet program. Growth time at 37 degreesC ranged from 4 h for samples at 10(7) cells/ml to 16 h for samples at 10 cells/ml. (C) 2002 Elsevier Science B.V All rights reserved.
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