Journal
ANALYTICA CHIMICA ACTA
Volume 482, Issue 1, Pages 81-89Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(03)00196-X
Keywords
pressurized hot water extraction; pressurized liquid extraction; medicinal plants; berberine; baicalein; glycyrrhizin; HPLC; extraction methods
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Pressurized hot water extraction (PHWE) using a laboratory made system was applied for the extraction of thermally labile and reasonably polar components such as berberine in coptidis rhizoma, glycyrrhizin in radix glycyrrhizae/liquorice and baicalein in scutellariae radix. PHWE was carried out dynamically at a flow of I ml/min, temperature between 95 and 140 degreesC, an applied pressure of 10-20 bar and extraction time of 40 min. Extraction by PHWE was found to give efficiencies comparable to Soxhlet extraction for baicalein in scutellariae radix and sonication for berberine in coptidis rhizoma, and glycyrrhizin in radix glycyrrhizae. Effects of ethanol added into the water used in PHWE were explored. Pressurized liquid extraction (PLE) with methanol as solvent was used for extraction of baicalein in scutellariae radix. The marker compounds present in the various medicinal plant extracts were determined by gradient elution HPLC. (C) 2003 Elsevier Science B.V. All rights reserved.
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