Journal
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
Volume 38, Issue 3, Pages 157-163Publisher
INFORMA HEALTHCARE
DOI: 10.3109/10731191003790406
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- Research Fund of Trakya University [TUBAP-821]
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Polyphenol oxidase (PPO, EC 1.14.18.1) was isolated from artichoke head (Cynara scolymus L.) by using 0.1 M Tris-HCl buffer (pH 7.0), concentrated by (NH4)(2)SO4 precipitation, and immobilized in copper-alginate beads. Immobilization yield was determined to be 70%. The cresolase and catecholase activities of enzyme immobilized at optimum immobilization conditions were found to be 13.3 and 670 U g beads min
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