Journal
FEBS LETTERS
Volume 541, Issue 1-3, Pages 115-120Publisher
WILEY
DOI: 10.1016/S0014-5793(03)00317-X
Keywords
dimethylallyl diphosphate; isopentenyl diphosphate; isoprenoid; LytB; 2-C-methyl-D-erythritol 4-phosphate pathway; (E)-4-hydroxy-3-methylbut-2-enyl diphosphate
Ask authors/readers for more resources
The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available