Journal
JOURNAL OF DERMATOLOGICAL SCIENCE
Volume 31, Issue 3, Pages 225-228Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/S0923-1811(03)00007-0
Keywords
beta-defensin; keratinocyte; celt line; differentiation; RT-PCR; antimicrobial peptide
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Background: Human beta-defensins (hBDs) belong to a group of antimicrobial peptide that are expressed in the epithelial cells. Objective: The present study investigated mRNA expression levels of the beta-defensins, hBD-1, -2 and -3, in human keratinocytes during differentiation in vitro. Methods: Immortalized keratinocyte cell lines, HaCaT and PHK16-0b, were used in this study; in order to stimulate differentiation, the Ca2+ concentration in the growth media was increased from 0.3 to 1.8 mM. Results: Four days after the increase, the expression levels of hBD-1 and 3 were increased in both cell lines, followed by an increase in the mRNA levels of the differentiation markers, involucrin and keratin 10. No increased expression of hBD-2 was observed. Conclusion: The results indicate that keratinocyte differentiation may stimulate hBD-1 and -3 expression in stratified squamous epithelia. (C) 2003 Japanese Society for Investigative Dermatology. Published by Elsevier Science Ireland Ltd. All rights reserved.
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