Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 69, Issue 5, Pages 3011-3014Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.69.5.3011-3014.2003
Keywords
-
Categories
Ask authors/readers for more resources
We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available