Journal
MOLECULAR THERAPY
Volume 7, Issue 5, Pages 640-648Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1525-0016(03)00064-9
Keywords
liposome; LPD; peptide vaccine; E7; HPV; cervical cancer
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Funding
- NCI NIH HHS [CA74918, CA74743] Funding Source: Medline
- NIAID NIH HHS [AI43916, AI48851] Funding Source: Medline
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Vaccination with antigenic peptides encoding tumor antigens has the potential to be an effective treatment for cancer. To induce tumor-specific cellular immune responses, a peptide antigen must be presented by antigen-presenting cells (APCs) to T-cells in the lymphatic tissues. Effective in vivo delivery of peptide antigens to APCs has been problematic. Here we use a model antigen from the HPV16 E7 protein to formulate LPD/E7 particles that upon iv administration are internalized by CD11c(+) and CD11b(+) cells in the marginal zone of the spleen. Either iv or sc vaccination with LPD/E7 particles induces E7-specific CTL responses stronger than those obtained using previously described liposome/peptide strategies and prevents the establishment of E7-expressing tumors. Furthermore, the administration of LPD/E7 particles to tumor-bearing mice caused complete tumor regression in 100% of the treated animals. Based on these studies, the entrapment of peptide antigens inside LPD particles may be an effective and generally applicable strategy for the enhancement of peptide vaccine potency.
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