Journal
JOURNAL OF FLUORESCENCE
Volume 13, Issue 3, Pages 267-276Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1023/A:1025046101335
Keywords
Metal-enhanced fluorescence; radiative decay engineering; fractal silver structures; increased photostabilities
Funding
- NCRR NIH HHS [P41 RR008119, P41 RR008119-12, P41 RR008119-13] Funding Source: Medline
- NIBIB NIH HHS [R01 EB000682, R01 EB000682-04, R01 EB000682-03] Funding Source: Medline
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Substantial increases in fluorescence emission from fluorophore-protein-coated fractal-like silver structures have been observed. We review two methods for silver fractal structure preparation, which have been employed and studied. The first, a roughened silver electrode, typically yielded a 100-fold increase in fluorophore emission, and the second, silver fractal-like structures grown on glass between two silver electrodes, produced a approximate to 500-fold increase. In addition, significant increases in probe photostability were observed for probes coated on the silver fractal like structures. These results further serve to compliment our recent work on the effects of nobel metal particles with fluorophores, a relatively new phenomenon in fluorescence we have termed both metal-enhanced fluorescence [1] and radiative decay engineering [2,3]. These results are explained by the metallic surfaces modifying the radiative decay rate (Gamma) of the fluorescent labels. We believe that this new silver-surface preparation, which results in ultrabright and photostable fluorophores, offers a new generic technology platform for increased fluorescence signal levels, with widespread potential applications to the analytical sciences, imaging, and medical diagnostics.
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