Study of the interaction between daunorubicin and human serum albumin, and the determination of daunorubicin in blood serum samples

The interactions between daunorubicin and human serum albumin (HSA) were studied using the spectrofluorimetric method. The binding constants of daunorubicin with HSA were obtained at different temperatures. The effects of various metal ions on the binding constants of daunorubicin with HSA were also studied. The optimum conditions of fluorometric determination of daunorubicin were studied and the developed method was successfully applied to the determination of daunorubicin in serum samples. (C) 2002 Elsevier Science B.V. All rights reserved.