Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 73, Issue 5, Pages 604-613Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0902450
Keywords
macrophage; shedding
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The study of the murine macrophage mannose receptor (MR) has been hampered by the lack of specific reagents. We have generated and characterized novel anti-MR monoclonal antibodies and used them to analyze MR expression in primary mouse macrophages (MO). In BioGel- and thioglycollate-elicited MO, interleukin (IL)-4 up-regulated total cell-associated MR (cMR), correlating with enhanced surface expression. We investigated the influence of IL-10, a well-characterized deactivator of MO function, on MR levels and observed that it had a similar effect to IL-4. In both cases, enhanced cMR levels translated into increased production of the soluble form of the receptor (sMR). We have demonstrated the presence of sMR in cultures of stable non-MO transductants expressing full-length MR, indicating that the proteolytic activity responsible for cMR cleavage is not MO-restricted. These data support a role for the MR in T helper cell type 2 cytokine-driven, immune responses and suggest a non-MO contribution to sMR production in vivo.
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