Journal
PHYTOCHEMICAL ANALYSIS
Volume 14, Issue 3, Pages 155-159Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/pca.697
Keywords
gas chromatography; leaf volatiles; mass spectrometry; methyl jasmonate; solid-phase microextraction
Ask authors/readers for more resources
Static headspace sampling with solid-phase microextraction has been used in combination with GC-FID and GC-MS for the specific enrichment, identification and quantification of volatile methyl jasmonate secreted by wounded leaves of Arabidopsis thaliana. The microsample method of analysis was found to be precise, accurate, sensitive and rapid. The detection limit of the procedure is 1.5 ppb (similar to1.3 ng) per injection, which is of adequate sensitivity to detect the natural baseline levels of methyl jasmonate (similar to10-100 ng/g) present in plant tissues. The method can be applied to most plants, requires a minimum of sample material, and shows the additional advantage that it is suitable for automation and could thus be used for high-throughput screening. Copyright (C) 2003 John Wiley Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available