Journal
BIOSENSORS & BIOELECTRONICS
Volume 18, Issue 5-6, Pages 613-619Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(03)00038-1
Keywords
BioMEMS; microdialysis; glucose; lactate; continuous monitoring; microfabrication
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The simultaneous on-line determination of glucose and lactate using a microdevice that consisted of a dialysis sampling system incorporated to the flow-through cell of a microfabricated biosensor array is presented. The fluidic connections between the different device's components were realized by subsequent processing of stacked dry resist layers on a plastic support that provided also the means for electric connections. The performance of the device was evaluated in vitro. The cross-talk effect on the downstream sensor was investigated and found to be negligible. Recoveries of over 95% for both analytes were achieved when flow rates of the perfusion fluid less than or equal to 0.5 mul/min were used. At this flow rate, the response time of the device was 2.4 min, which is acceptable for on-line analysis. The linear response concentration range extended up to 30 mM for glucose and 15 mM for lactate. Interference from electroactive species such as ascorbic acid, 2-acetamidophenol and uric acid, was minimal (less than 5% increase in biosensors signal for all substances tested). In addition, the device presented long-term run stability both in buffer and serum samples. (C) 2003 Elsevier Science B.V. All rights reserved.
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