Journal
BIOLOGY OF REPRODUCTION
Volume 68, Issue 5, Pages 1779-1786Publisher
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.102.009407
Keywords
assisted reproductive technology
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Funding
- NICHD NIH HHS [5U01HD38227-03] Funding Source: Medline
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Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from the B6D2F1 strain isolated in an ECTA-supplemented Tris-HCI buffer were dried using three different drying rates and were stored for 18-24 h at 4degreesC. The mean final moisture content was <5% for all the protocols. After intracytoplasmic sperm injection (ICSI), the mean blastocyst formation rates were 64%, 58%, and 35% using the rapid-, moderate-, and slow-drying protocols, respectively. The slow-drying protocol resulted in a rate of development significantly lower than that observed using rapid- and moderate-drying protocols and indicated that a slower drying rate may be detrimental to the DNA integrity of mouse sperm. The transfer of 85 two- or four-cell embryos that were produced using rapidly desiccated sperm resulted in 11 fetuses (13%) on Day 15 compared with the production of 34 fetuses (40%) produced using the transfer of 86 two- or four-cell embryos that were produced using fresh sperm (P < 0.05). The results demonstrate the feasibility of using a convective drying protocol for the successful desiccation of mouse sperm and identifies some of the important parameters required for optimization of the procedure.
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