Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 22, Issue 5, Pages 273-+Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/S0165-9936(03)00508-9
Keywords
mass spectrometry (MS); protein identification; proteome; proteomics; two-dimensional gel electrophoresis (2DE)
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Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key components of current proteomics technology. The purpose of this review is to give an overview of the basic concepts of 2DE-based proteomics methodology, to discuss the advantages and limitations of the approach, and to highlight some recent advancements in 2DE-based technology. (C) 2003 Published by Elsevier Science B.V.
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