Journal
ARTHRITIS AND RHEUMATISM
Volume 64, Issue 9, Pages 2975-2985Publisher
WILEY-BLACKWELL
DOI: 10.1002/art.34504
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Funding
- NIH [T32-AI-007413, HL-088419, P30-AR-48310, P60-DK-20572, P30-DK-089503]
- Anthony Gramer Fund in Inflammation Research
- Department of Veterans Affairs
- Frederick Huetwell and William Robinson, MD, Professorship in Rheumatology at the University of Michigan
- Molecular Phenotyping Core, Michigan Nutrition and Obesity Research Center, University of Michigan
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Objective. Patients with systemic lupus erythematosus (SLE) have a notable increase in atherothrombotic cardiovascular disease (CVD) which is not explained by the Framingham risk equation. In vitro studies indicate that type I interferons (IFNs) may play prominent roles in increased CV risk in SLE. However, the in vivo relevance of these findings, with regard to the development of CVD, has not been characterized. This study was undertaken to examine the role of type I IFNs in endothelial dysfunction, aberrant vascular repair, and atherothrombosis in murine models of lupus and atherosclerosis. Methods. Lupus-prone New Zealand mixed 2328 (NZM) mice and atherosclerosis-prone apolipoprotein E-knockout (apoE(-/-)) mice were compared to mice lacking type I IFN receptor (INZM and apoE(-/-)IFNAR(-/-) mice, respectively) with regard to endothelial vasodilatory function, endothelial progenitor cell (EPC) function, in vivo neoangiogenesis, plaque development, and occlusive thrombosis. Similar experiments were performed using NZM and apoE(-/-) mice exposed to an IFN alpha-containing or empty adenovirus. Results. Loss of type I IFN receptor signaling improved endothelium-dependent vasorelaxation, lipoprotein parameters, EPC numbers and function, and neoangiogenesis in lupus-prone mice, independent of disease activity or sex. Further, acute exposure to IFN alpha impaired endothelial vasorelaxation and EPC function in lupus-prone and non-lupus-prone mice. Decreased atherosclerosis severity and arterial inflammatory infiltrates and increased neoangiogenesis were observed in apoE(-/-)IFNAR(-/-) mice, compared to apoE(-/-) mice, while NZM and apoE(-/-) mice exposed to IFN alpha developed accelerated thrombosis and platelet activation. Conclusion. These results support the hypothesis that type I IFNs play key roles in the development of premature CVD in SLE and, potentially, in the general population, through pleiotropic deleterious effects on the vasculature.
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