Inflammation-Independent Defective Early B Cell Tolerance Checkpoints in Rheumatoid Arthritis

Objective. Rheumatoid arthritis (RA) patients who have never received treatment for RA have been found to have defective early B cell tolerance checkpoints, resulting in impaired removal of developing autoreactive B cells. However, it is unclear whether these defects in B cell tolerance checkpoints are a primary aspect of the disease or are the result of ongoing inflammatory processes in these patients. The aim of this study was to assess the impact of standard immunosuppressive treatments, methotrexate and antitumor necrosis factor alpha (anti-TNF alpha) agents, on early B cell tolerance checkpoints in RA patients. Methods. Blood samples were obtained from RA patients before and after treatment with methotrexate and/or anti-TNF alpha agents. B cells were tested pre- and posttherapy for reactivity of recombinant antibodies cloned from single B cells, which allowed us to determine the evolution of the frequency of autoreactive clones in the mature naive B cell compartment in RA patients before and after treatment. B cells from healthy donors were used as controls. Results. Posttreatment frequencies of autoreactive mature naive B cells were elevated in the blood of RA patients. Nevertheless, the frequencies after treatment remained similar to those observed in the same patients before treatment. Conclusion. Despite the achievement of clinical improvement in RA patients following treatment with methotrexate and/or anti-TNF alpha agents, these therapies did not correct the accumulation of peripheral autoreactive mature naive B cells in these patients, suggesting that inflammation is not responsible for the defective early B cell tolerance checkpoints in RA.