Journal
BIOSENSORS & BIOELECTRONICS
Volume 18, Issue 5-6, Pages 547-553Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(03)00026-5
Keywords
biosensors; heavy metals; phytochelatins; capacitance
Ask authors/readers for more resources
A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)(20)Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Ph2+, Cu2+ and Zn2+ ions in concentration range of 100 fM- 10 mM, and the order of sensitivity was S-Zn > S-Cu > S-Hg much greater than S-Cd congruent to S-Pb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days. (C) 2003 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available