Journal
JOURNAL OF MICROSCOPY-OXFORD
Volume 210, Issue -, Pages 158-165Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2818.2003.01191.x
Keywords
3D reconstruction; collagen fibre; confocal microscopy; ECM; tracking algorithm
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The geometrical structure of fibrous extracellular matrix (ECM) impacts on its biological function. In this report, we demonstrate a new algorithm designed to extract quantitative structural information about individual collagen fibres (orientation, length and diameter) from 3D backscattered-light confocal images of collagen gels. The computed quantitative data allowed us to create surface-rendered 3D images of the investigated sample.
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