Journal
SCIENCE
Volume 300, Issue 5620, Pages 805-808Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1082320
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Funding
- NIGMS NIH HHS [R01 GM045443, GM45443] Funding Source: Medline
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A major pathway of eukaryotic messenger RNA ( mRNA) turnover begins with deadenylation, followed by decapping and 5' to 3' exonucleolytic decay. We provide evidence that mRNA decapping and 5' to 3' degradation occur in discrete cytoplasmic foci in yeast, which we call processing bodies ( P bodies). First, proteins that activate or catalyze decapping are concentrated in P bodies. Second, inhibiting mRNA turnover before decapping leads to loss of P bodies; however, inhibiting turnover at, or after, decapping, increases the abundance and size of P bodies. Finally, mRNA degradation intermediates are localized to P bodies. These results de. ne the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation.
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