4.5 Article

Identification of Primula fatty acid Δ6-desaturases with n-3 substrate preferences

Journal

FEBS LETTERS
Volume 542, Issue 1-3, Pages 100-104

Publisher

WILEY
DOI: 10.1016/S0014-5793(03)00358-2

Keywords

cytochrome b(5) fusion; desaturase; 7-linolenic acid; polyunsaturated fatty acid

Funding

  1. Biotechnology and Biological Sciences Research Council [BBS/E/C/00004156] Funding Source: researchfish
  2. Biotechnology and Biological Sciences Research Council [BBS/E/C/00004156] Funding Source: Medline

Ask authors/readers for more resources

Fatty acid Delta(6)-desaturation, the first committed step in C-20 polyunsaturated fatty acid biosynthesis, is generally considered not to discriminate between n-3 and n-6 substrates. We previously identified higher plant species that showed preferential Delta(6)-desaturation of n-3 C-18 fatty acid substrates. A polymerase chain reaction-based approach was used to isolate 'front-end' cytochrome b(5) fusion desaturases from Primula vialii Franchet and Primula farinosa L. Functional analysis in Saccharomyces cerevisiae identified fatty acid Delta(6)-desaturases with a strong specificity for the n-3 substrate alpha-linolenic acid (18: 3Delta(9,12,15)). These results indicate that the accumulation of octadecatetraenoic acid (18:4Delta(6,9,12,15)) in planta is due to the activity of a novel n-3-specific fatty acid Delta(6)-desaturase. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available