Journal
FEBS LETTERS
Volume 542, Issue 1-3, Pages 17-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00328-4
Keywords
melioidosis; oxidative stress; catalase-peroxidase; non-specific DNA binding protein; oxyR; gene regulation
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Homologues of the catalase-peroxidase gene katG and the gene for the non-specific DNA binding protein dpsA were identified downstream of oxyR in Burkholderia pseudomallei. Northern experiments revealed that both katG and dpsA are co-transcribed during oxidative stress. Under conditions where the katG promoter is not highly induced, dpsA is transcribed from a second promoter located within the katG-dpsA intergenic region. A katG insertion mutant was found to be hypersensitive to various oxidants. Analysis of katG expression in the oxyR mutant indicates that OxyR is a dual function regulator that represses the expression of katG during normal growth and activates katG during exposure to oxidative stress. Both reduced and oxidized OxyR were shown to bind to the katG promoter. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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