Journal
APPLIED CATALYSIS B-ENVIRONMENTAL
Volume 42, Issue 2, Pages 213-221Publisher
ELSEVIER
DOI: 10.1016/S0926-3373(02)00238-2
Keywords
horseradish peroxidase; lignin peroxidase; enzymatic oxidations; N-demethylation; methylene blue; azure B; azure C
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We have previously shown that the oxidation of methylene blue (MB) or azure B (AB) by Phanerochaete chrysosporium lignin peroxidase (LiP) (class 11) occurs via stepwise N-demethylations followed by aromatic ring cleavage under selective reaction conditions related to methylene blue:H2O2 or azure B:H2O2 stoichiometry. In this work, we compare the oxidation of the same dyes by horseradish peroxidase (HRP) of plant origin (class 111) and compare UP and HRP reactions and products. Results show HRP is able to N-demethylate both dyes, but exhibits much slower reaction kinetics than UP and requires higher H2O2 concentrations. Product yields are also different for HRP, and contrary to LiP, HRP is unable to achieve aromatic ring cleavage. Azure C (AC), which is formed by sequential oxidation of either MB or AB, was a major reaction product in HRP-mediated reactions. Yields of AC up to 60% were obtained, suggesting a potential enzymatic route for AC production that compares quite favorably to the chemical route yield of 35%. (C) 2002 Elsevier Science B.V. All rights reserved.
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