Journal
MOLECULAR BRAIN RESEARCH
Volume 113, Issue 1-2, Pages 139-142Publisher
ELSEVIER
DOI: 10.1016/S0169-328X(03)00125-6
Keywords
glucose sensing; hypothalamus; hindbrain; real time RT-PCR
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Glucokinase (GK), glucose transporter GLUT2, sulfonylurea receptor-1 (SUR1), glucagon-like peptide-1 receptor (GLP-1R) and neuropeptide Y (NPY) have been proposed to be involved in central glucose sensing or regulation of food intake. In this study, we combined tissue micropunch and real time reverse transcription polymerase chain reaction (RT-PCR), and measured GK, GLUT2, SUR1, GLP-1R and NPY mRNA expression in discrete areas in the hypothalamus and the hindbrain. (C) 2003 Elsevier Science B.V. All rights reserved.
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