Journal
NUCLEIC ACIDS RESEARCH
Volume 31, Issue 10, Pages 2622-2629Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkg353
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Binding of actinomycin D (ActD) to the seemingly single-stranded DNA (ssDNA) oligomer 5'-CCGTT(3) GTGG-3' has been studied in solution using high-resolution nuclear magnetic resonance (NMR) techniques. A strong binding constant (8 x 10(6) M-1) and high quality NMR spectra have allowed us to determine the initial DNA structure using distance geometry as well as the final ActD-5'-CCGTT(3) GTGG-3' complex structure using constrained molecular dynamics calculations. The DNA oligomer 5'-CCGTT(3)GTGG-3' in the complex forms a hairpin structure with tandem G.T mismatches at the stem region next to a loop of three stacked thymine bases pointing toward the major groove. Bipartite (TO)-O-2-GH1 and (TO)-O-2-G(2)NH(2) hydrogen bonds were detected for the G.T mismatches that further stabilize this unusual DNA hairpin. The phenoxazone chromophore of ActD intercalates nicely between the tandem G.T mismatches in essentially one major orientation. Additional hydrophobic interactions between the ActD quinoid amino acid residues with the loop T5-T6-T7 backbone protons were also observed. The hydrophobic G-phenoxazone-G interaction in the ActD-5'-CCGTT(3)GTGG-3' complex is more robust than that of the classical ActD- 5'-CCGCT(3)GCGG-3' complex, consistent with the roughly 2-fold stronger binding of ActD to the 5'-CCGTT(3)GTGG-3' sequence than to its 5'-CCG CT(3)GCGG-3' counterpart. Stabilization by ActD of a hairpin containing non-canonical stem base pairs further strengthens the notion that ActD or other related compounds may serve as a sequence- specific ssDNA-binding agent that inhibits human immunodeficiency virus (HIV) and other retroviruses replicating through ssDNA intermediates.
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