Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 44, Issue 2, Pages 225-230Publisher
OXFORD UNIV PRESS
DOI: 10.1016/S0168-6496(03)00031-X
Keywords
Na+-dependent NADH oxidase; marine bacteria; primary Na+ pump; fish pathogen; Vibrio anguillarum
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The activity of membrane-bound NADH oxidase of Vibrio anguillarum M93 (serotype J-O-1), which causes vibriosis in freshwater area was activated by Na+ in the same manner as other marine Vibrios. However, in addition to Na+, K+ was also found to positively enhance the NADH oxidase activity of strain M93. This tendency has not been recognized in other marine Vibrios. Furthermore, the Na+-dependent NADH oxidase of strain M93 required less Na+ (0.1 M) for its maximum activity than those of other Vibrios such as Vibrio alginolyticus and 'Vibrio angustum' S14, which were in the range of 0.4 M NaCl, similar as seawater. Destruction of H+ motive force by a proton conductor carbonylcyanide m-chlorophenylhydrazone (CCCP) revealed high dependency of V anguillarum on the primary H+ pump. Even at pH 8.5, V anguillarum strains other than serotype O-4 could not grow well with the addition of CCCP. In contrast, marine-type Vibrios such as V. alginolyticus and V. angustum S14 can grow well at pH 8.5 even with the addition of CCCP. The lower requirement for Na+ in V anguillarum probably reflects the salinity of their original habitats. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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