Journal
BIOCHEMICAL JOURNAL
Volume 372, Issue -, Pages 241-246Publisher
PORTLAND PRESS
DOI: 10.1042/BJ20021765
Keywords
conformational stability; glutathione; thioredoxin fold
Categories
Ask authors/readers for more resources
The thioredoxin-like fold has betaalphabetaalphabetaalpha topology, and most proteins/domains with this fold have a topologically conserved cis-proline residue at the N-terminus of beta-strand 3. This residue plays an important role in the catalytic function and stability of thioredoxin-like proteins, but is reported not to contribute towards the stability of glutathione S-transferases (GSTs) [Allocati, Casalone, Masulli, Caccarelli, Carletti, Parker and Di Ilio (1999) FEBS Lett. 445, 347-350]. In order to further address the role of the cis-proline in the structure, function and stability of GSTs, cisPro-56 in human GST (hGST) A1-1 was replaced with a glycine, and the properties of the P56G mutant were compared with those of the wild-type protein. Not only was the catalytic function of the mutant dramatically reduced, so was its conformational stability, as indicated by equilibrium unfolding and unfolding kinetics experiments with urea as denaturant. These findings are discussed in the context of other thioredoxin-like proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available