Journal
EXPERIMENTAL CELL RESEARCH
Volume 286, Issue 1, Pages 12-21Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0014-4827(03)00102-2
Keywords
protilin; nuclear distribution; pre-mRNA splicing; Sm proteins
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Profilin is one of the major components controlling actin polymerization. Here, profilin I was located in fibroblasts and HeLa cells by the use of two different sets of affinity-purified antibodies. Both antibody preparations labeled nuclei in a speckle-like pattern and displayed extensive colocalization with small nuclear ribonucleoprotein particle (snRNP)-core proteins and p80 coilin-containing Cajal bodies. Treatment with actinomycin D Led to Largely similar reorganizations of snRNPs and profilin, while profilin and Cajal bodies separated under these conditions. One of the profilin antibodies interfered with pre-mRNA splicing in vitro, further indicating a role for profilin during pre-mRNA processing. (C) 2003 Elsevier Science (USA). All rights reserved.
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