Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 13, Issue 10, Pages 1653-1656Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0960-894X(03)00280-4
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The water soluble fluorescein-based ligand 1 forms a non-fluorescent complex with Cu2+. This complex serves as a fluorescent sensor for amino acids in the 10(-3) M Concentration range. Since the signal response is very fast, the sensor can be used to detect the hydrolytic activity of various proteases (trypsin, chymotrypsin, subtilisin) on bovine serum albumin as a whole protein substrate, and more generally to follow reactions releasing or removing free amino acids, in real time. (C) 2003 Elsevier Science Ltd. All rights reserved.
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