Journal
ONCOGENE
Volume 22, Issue 22, Pages 3424-3430Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1206413
Keywords
Kruppel-like factor 4; tumorigenecity; RKO; colorectal cancer
Funding
- NCI NIH HHS [R01 CA084197, R01 CA084197-04, CA84197] Funding Source: Medline
- NIDDK NIH HHS [K08 DK059970, R01 DK052230, R01 DK052230-06, DK52230, DK59970] Funding Source: Medline
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Kruppel-like factor 4 (KLF4) is a zinc-finger-containing transcription factor, the expression of which is enriched in the postmitotic cells of the intestinal epithelium. KLF4 is a target gene of the tumor suppressor adenomatous polyposis coli (APC). We sought to determine the role of KLF4 in suppressing the tumorigenecity of RKO colon cancer cells, which do not express KLF4. We utilized an established system in RKO cells, in which an inducible promoter controls expression of KLF4. Four independent assays were used to assess the effects of KLF4 induction on tumor cells. We find that KLF4 overexpression reduces colony formation, cell migration and invasion, and in vivo tumorigenecity. The mechanism of action of KLF4 does not involve apoptosis. These findings, along with our previous findings that KLF4 induces G1/S arrest, suggest that KLF4 is a cell cycle checkpoint protein that can reduce tumorigenecity of colon cancer cells.
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