Journal
NUCLEIC ACIDS RESEARCH
Volume 31, Issue 11, Pages 2944-2951Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkg392
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Funding
- NIGMS NIH HHS [R01 GM039799, R01 GM065988, R01 GM39799, R01 GM65988] Funding Source: Medline
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Distamycin binds the minor groove of duplex DNA at AT-rich regions and has been a valuable probe of protein interactions with double-stranded DNA. We find that distamycin can also inhibit protein interactions with G-quadruplex (G4) DNA, a stable four-stranded structure in which the repeating unit is a G-quartet. Using NMR, we show that distamycin binds specifically to G4 DNA, stacking on the terminal G-quartets and contacting the flanking bases. These results demonstrate the utility of distamycin as a probe of G4 DNA-protein interactions and show that there are (at least) two distinct modes of protein-G4 DNA recognition which can be distinguished by sensitivity to distamycin.
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