Journal
FOOD ADDITIVES AND CONTAMINANTS
Volume 20, Issue 6, Pages 528-534Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/0265203031000098696
Keywords
beta-lactams; antibiotics; milk; HPLC-MS
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A procedure for the simultaneous determination of the residues of seven beta-lactam antibiotics (penicillin G, ampicillin, oxacillin, amoxicillin, dicloxacillin, cephalexin, cephapirin) in bovine raw milk using tandem liquid chromatography mass spectrometry (LC-MS/MS) is described. The antibiotics were extracted by an acetic acid solution after centrifugation and filtration. The beta-lactams were separated using reversed-phase liquid chromatography. A triple quadrupole mass spectrometer was used in positive-ion mode as a detector via a Turbo Ionspray interface for electrospray ionization (ESI). The limits of detection and quantitation of the method were below the legal tolerances, except for ampicillin. The method was used to confirm 53 samples found positive by a microbial method (Delvotest SP(R)) at the Istituto Zooprofilattico Sperimentale per la Lombardia e l'Emilia Romagna of Brescia during 2001. Penicillin G was found in 26 samples at concentrations ranging from less than 4 to 6240 +/- 550 mug l(-1). Amoxicillin was found in three samples at concentrations ranging from 8.5 +/- 0.1 to 53.7 +/- 2.3 mug l(-1). Cephapirin was found in two samples at 5.7 +/- 0.1 and 6.4 +/- 0.3 mug l(-1).
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