Journal
BIOCHEMICAL SOCIETY TRANSACTIONS
Volume 31, Issue -, Pages 497-501Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BST0310497
Keywords
cytochrome P450 reductase; electron transfer; flavoprotein; kinetics
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Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.
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