Journal
EUROPEAN JOURNAL OF BIOCHEMISTRY
Volume 270, Issue 12, Pages 2671-2679Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1432-1033.2003.03644.x
Keywords
glycoconjugate; MALDI-TOF-MS; NMR; Treponema medium
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Glycoconjugates are distributed on the cell surfaces of some small-sized treponemes and have been reported to be completely different from lipopolysaccharides. We separated a glycoconjugate fraction from Treponema medium ATCC 700293, a medium-sized oral spirochete, to assess its immunobiological activities and elucidate the chemical structure of its polysaccharide part using phenol/water extraction, hydrophobic chromatography, and gel filtration. The glycoconjugate showed negligible or weak endotoxic and immunobiological properties. The chemical structure of the polysaccharide part was shown by two-dimensional NMR and MALDI-TOF-MS to be a tetrasaccharide backbone with two amino acids: [-->4)beta-d-Glcp NAc3NAcA(1-->4)beta-d-Manp NAc3NAOrn(1-->3)beta-d-Glcp NAc(1-->3)alpha-D-Fucp 4NAsp(1-->] where GlcNAc3NAcA is 2,3-diacetamido-2,3-dideoxyglucuronic acid, ManNAc3NAOrn is N (delta) -(2-acetamido-3-amino-2,3-dideoxymannuronyl)ornithine, and Fuc4NAsp is 4-(alpha-aspartyl)amino-4,6-dideoxygalactose.
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