Journal
PLANT JOURNAL
Volume 34, Issue 5, Pages 733-739Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-313X.2003.01759.x
Keywords
transcriptional repressor; EAR-motif; dominant repression; transcription factor; chimeric repressor; genetic redundancy
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The redundancy of genes for plant transcription factors often interferes with efforts to identify the biologic functions of such factors. We show here that four different transcription factors fused to the EAR motif, a repression domain of only 12 amino acids, act as dominant repressors in transgenic Arabidopsis and suppress the expression of specific target genes, even in the presence of the redundant transcription factors, with resultant dominant loss-of-function phenotypes. Chimeric EIN3, CUC1, PAP1, and AtMYB23 repressors that included the EAR motif dominantly suppressed the expression of their target genes and caused insensitivity to ethylene, cup-shaped cotyledons, reduction in the accumulation of anthocyanin, and absence of trichomes, respectively. This (c) under bar himeric (re) under bar pressor (s) under bar ilencing (t) under bar echnology (CRES-T), exploiting the EAR-motif repression domain, is simple and effective and can overcome genetic redundancy. Thus, it should be useful not only for the rapid analysis of the functions of redundant plant transcription factors but also for the manipulation of plant traits via the suppression of gene expression that is regulated by specific transcription factors.
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