Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 12, Pages 919-925Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1024071514438
Keywords
baculovirus; enterovirus 71; insect cell; virus aggregation; virus-like particle
Categories
Ask authors/readers for more resources
The assembly of enterovirus requires the cleavage of P1 polyprotein by protease 3CD into individual structural proteins. Two recombinant baculoviruses were constructed to encode P1 and 3CD of enterovirus 71 (EV71), respectively. The expressed 3CD successfully cleaved P1 in vitro and in vivo. Also, the co-infection in insect cells resulted in crystalline virus-like particle structures morphologically resembling the authentic EV71 aggregates, which are reported for the first time.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available