Journal
NEPHROLOGY DIALYSIS TRANSPLANTATION
Volume 18, Issue 6, Pages 1108-1114Publisher
OXFORD UNIV PRESS
DOI: 10.1093/ndt/gfg108
Keywords
hypoglycosylated IgA1; IgA nephropathy; isoelectric focusing; alpha 2,3(O)sialyltransferase; secretory IgA; tonsillectomy
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Background. There are many reports of incompletely glycosylated O-linked oligosaccharides on the IgAl hinge region in certain IgA nephropathy patients. In addition, other reports have noted a relationship between tonsillectomy and IgA nephropathy. Methods. Immunoglobulins from extracts of tonsillectomized tissue and other sources were analysed by isoelectric focusing (IEF) and by enzyme-linked immunosorbent assay (ELISA). Results. The IEF profile of tonsillar IgA differed from that of serum IgA and it was enriched in cationic IgA. However, extracts from tonsillitis controls and IgA nephropathy patients exhibited profiles that were very similar. Enzymatic removal of sialic acid induced a shift of the peaks to the cathode side. The profiles of IgA from treated tonsillar extract and treated serum were closely overlapped. In addition, asialo Galbeta1, 3GalNAc was clearly present in cationic IgA from tonsillar extract and in aberrant IgAl from serum following enzymatic transfer of sialic acid to IgAl. Serum IgA also contained partly sialylated IgAl. Quantitative analysis of IgA and IgG in the extracts indicated that IgA was significantly higher, whereas IgG was significantly lower in IgA nephropathy patients. Conclusions. We found that the IgAl produced in tonsillar tissue differed from serum IgAl. Furthermore, an overproduction of asialo IgAl resulted from the disordered balance between IgA- and IgG-producing cells in the tonsils from the IgA nephropathy patient. Although it is unclear how such asialo IgAl molecules are transferred from tonsil tissue to serum, a tonsillar source may produce a few micrograms of aberrant IgAl that then appears in serum.
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