Journal
CHEMICAL PHYSICS
Volume 291, Issue 1, Pages 97-114Publisher
ELSEVIER
DOI: 10.1016/S0301-0104(03)00187-3
Keywords
triplet quantum yield; intersystem-crossing; riboflavin; flavin mononucleotide; LOV1 domain; alga Chlamydomonas reinhardtii
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The quantum yields of triplet formation, phi(T), of riboflavin, and of flavin mononucleotide (FMN) bound to wild-type LOV1 domain (LOV1-WT) or bound to a mutated LOV1 domain (LOV1-C57S, cysteine 57 replaced by serine) of the photo-receptor Phot1 from the green alga Chlamydomonas reinhardtii are determined by picosecond laser double-pulse excitation and time-resolved fluorescence detection. The determined quantum yields of triplet formation are phi(T) 0.375 +/- 0.05 for riboflavinin in aqueous solution at pH = 7, phi(T), = 0.225 +/- 0.04 for FMN in LOV1-C57S in aqueous solution at pH = 8, and phi(T) = 0.255 +/- 0.04 for FMN in LOV1-WT in aqueous solution at pH = 8. (C) 2003 Elsevier Science B.V. All rights reserved.
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