Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 376, Issue 3, Pages 392-398Publisher
SPRINGER-VERLAG BERLIN
DOI: 10.1007/s00216-003-1909-y
Keywords
optical sensor; coumarin derivative; fluorescence quenching; covalent immobilization; nitrofurazone
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A coumarin derivative 4-methyl-8-methylacrylamide-2H, 5H-pyrano [3, 2-C] benzpyran 2, 5-dione (MMPBD) has been synthesized as a fluorescent carrier for preparing an optical chemical sensor. The carrier is immobilized on a quartz glass plate surface treated with a silanizing agent to prevent the leakage of the dye. This MMPBD sensor can be utilized for a nitrofurazone (NF) assay based on fluorescence quenching. The sensor shows good repeatability, a long lifetime and a fast response of less then 50 s. NF can be determined in the range between 1.0x10(-6)-1.0x10(-3) mol L-1 with a detection limit Of 8.0x10(-7) mol L-1 at pH 7.0.
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