Journal
JOURNAL OF RADIATION RESEARCH
Volume 44, Issue 2, Pages 185-189Publisher
JAPAN RADIATION RESEARCH SOC
DOI: 10.1269/jrr.44.185
Keywords
urine; 8-hydroxydeoxyguanosine; HPLC-ECD; oxidative DNA damage
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A new method was developed to analyze urinary 8-hydroxydeoxyguanosine (8-OH-dG) by high-performance liquid chromatography (HPLC) coupled to air electrochemical detector (ECD). This method is unique because (i) urine is first fractionated by anion exchange chromatography (polystyrene-type resin with quaternary ammonium group, sulfate form) before analysis by reverse phase chromatography; and (ii) the 8-OH-dG fraction in the first HPLC is precisely and automatically collected based oil the added ribonucleoside 8-hydroxyguanosine marker peak, which elutes 4-5 min earlier. Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion.
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