Journal
GENOMICS
Volume 81, Issue 6, Pages 543-555Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0888-7543(03)00045-4
Keywords
human lymphoid cells; CEM; apoptosis; glucocorticoid sensitivity/resistance; microarray analysis; novel genes; gene expression profile; data mining
Funding
- NCI NIH HHS [R01 CA041407-14, R01 CA041407] Funding Source: Medline
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Three closely related clones of leukemic lymphoid CEM cells were compared for their gene expression responses to the glucocorticoid dexamethasone (Dex). All three contained receptors for Dex, but only two responded by undergoing apoptosis. After a time of exposure to Dex that ended late in the interval preceding onset of apoptosis, gene microarray analyses were carried out. The results indicate that the expression of a limited, distinctive set of genes was altered in the two apoptosis-prone clones, not in the resistant clone. That clone showed altered expression of different sets of genes, suggesting that a molecular switch converted patterns of gene expression between the two phenotypes: apoptosis-prone and apoptosis-resistant. The results are consistent with the hypothesis that altered expression of a distinctive network of genes after glucocorticoid administration ultimately triggers apoptosis of leukemic lymphoid cells. The altered genes identified provide new foci for study of their role in cell death. (C) 2003 Elsevier Science (USA). All rights reserved.
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