Journal
PLANT JOURNAL
Volume 34, Issue 5, Pages 741-750Publisher
WILEY
DOI: 10.1046/j.1365-313X.2003.01758.x
Keywords
activation tagging; Arabidopsis; gene family; ASYMMETRIC LEAVES 2; ASL/LBD; AS2/LOB domain
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In a screen for morphological mutants from the T-1 generation of approximately 50 000 activation-tagging lines, we isolated four dominant mutants that showed hyponastic leaves, downward-pointing flowers and decreased apical dominance. We designated them isoginchaku (iso) . The iso-1D and iso-2D are allelic mutants caused by activation of the AS2 gene. The T-DNAs were inserted in the 3' downstream region of AS2 . Iso-3D and iso-4D are the other allelic mutants caused by activation of the ASL1/LBD36 gene. These two genes belong to the AS2 family that is composed of 42 genes in Arabidopsis . The only recessive mutation isolated from this gene family was of AS2 , which resulted in a leaf morphology mutant. Applying reverse genetics using a database of activation-tagged T-DNA flanking sequences, we found a dominant mutant that we designated peacock1-D (pck1-D) in which the ASL5/LBD12 gene was activated by a T-DNA. The pck1-D mutants have lost apical dominance, have epinastic leaves and are sterile. These results strongly suggest that activation tagging is a powerful mutant-mining tool especially for genes that make up a gene family.
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