Journal
JOURNAL OF BACTERIOLOGY
Volume 185, Issue 11, Pages 3480-3483Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.185.11.3480-3483.2003
Keywords
-
Categories
Funding
- NIAID NIH HHS [AI30492, R01 AI030492, R37 AI030492] Funding Source: Medline
Ask authors/readers for more resources
An essential component of type III secretion systems (TTSS) is a supramolecular structure termed the needle complex. In Salmonella enterica, at least four proteins make up this structure: InvG, PrgH, PrgK, and PrgI. Another protein, PrgJ, is thought to play a role in the assembly of this structure, but its function is poorly understood. We have analyzed the expression and localization of PrgJ and the needle protein PrgI in different S. enterica serovar Typhimurium mutant strains. We found that the levels of PrgI and PrgJ were significantly reduced in a TTSS-deficient invA mutant strain and that the decreased levels were due to protein instability. In addition, we found that PrgJ, although associated with the needle complex in wild-type S. enterica serovar Typhimurium, was absent from needle complexes obtained from an invJ mutant strain, which exhibits very long needle substructures. We suggest that PrgJ is involved in capping the needle substructure of the needle complex.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available