Journal
JOURNAL OF MEDICAL VIROLOGY
Volume 70, Issue 2, Pages 205-211Publisher
WILEY-LISS
DOI: 10.1002/jmv.10379
Keywords
nanoparticle; nano-amplification; gene detection; hepatitis virus
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A visual gene-detecting technique using nanoparticle-supported gene probes is described. With the aid of gold nanoparticle-supported T-end-mercapto-derivatized oligonucleotide serving as detection probe, and 5'-end-amino-derivatized oligonucleotide immobilized on glass surface acting as capturing probe, target DNA was detected visually by sandwich hybridization based on highly sensitive nano-amplification and silver staining. Different genotypes of Hepatitis B and C viruses in the serum samples from infected patients were detected using homemade HBV, HCV, and HBV/HCV gene chips by the gold/silver nanoparticle staining amplification method. The present visual gene-detecting technique may avoid limitations with the reported methods, for its high sensitivity, good specificity, simplicity, speed, and cheapness. This technique has potential applications in many fields, especially in multi-gene detection gene chips coupled with the detection will find applications in clinic. Additionally, resonance Rayleigh light scattering (RLS) spectroscopy is used, for the first time, to judge and monitor the immobilization of gene probes on gold nanoparticle surfaces.
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