Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 41, Issue 6-7, Pages 649-655Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/S0981-9428(03)00077-9
Keywords
Arabidopsis thaliana; gene expression; glutamate synthase; light regulation; nitrogen; promoter analysis; transgenic Nicotiana tabacum
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The GLU1 promoter for Fd-glutamate synthase (Fd-GOGAT, EC 1.4.1.7) of Arabidopsis thaliana (ecotype Columbia) confers the expression of the beta-glucuronidase (GUS) reporter gene on transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) transformed with the GLU1 promoter-GUS construct. Histochemical analysis reveals that GUS expression is associated with mesophyll and vascular tissue of 14-d-old tobacco seedlings. Red light substitutes for white light and induces a 2-fold increase in the GUS expression associated with mesophyll, veins and vascular tissue. Sucrose also serves as a signal to induce GUS expression in mesophyll and veins of cotyledons. Mature leaves, adapted to the dark for 3 d, conserves the red light- and white light-dependent inductions of GUS activity, while GUS expression is repressed by white light in roots. The mesophyll-located expression of the GLU1 promoter suggests that Fd-glutamate synthase has a function in the photorespiratory ammonium cycling and primary ammonium assimilation. The distinct location of GLU1 promoter expression in the vascular tissue supports the view that Fd-glutamate synthase synthesises glutamate for intracellular transport of glutamine and glutamate. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
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