Journal
EMBO JOURNAL
Volume 22, Issue 11, Pages 2668-2678Publisher
WILEY
DOI: 10.1093/emboj/cdg256
Keywords
Candida albicans; filamentous growth; haploinsufficiency
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Funding
- NIGMS NIH HHS [R01 GM037049, GM37049] Funding Source: Medline
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Candida albicans is the most prevalent human fungal pathogen. Here, we take advantage of haploinsufficiency and transposon mutagenesis to perform large-scale loss-of-function genetic screen in this organism. We identified mutations in 146 genes that affect the switch between its single-cell (yeast) form and filamentous forms of growth; this switch appears central to the virulence of C.albicans. The encoded proteins include those involved in nutrient sensing, signal transduction, transcriptional control, cytoskeletal organization and cell wall construction. Approxim ately one-third of the genes identified in the screen lack homologs in Saccharomyces cerevisiae and other model organisms and thus constitute candidate antifungal drug targets. These results illustrate the value of performing forward genetic studies in bona fide pathogens.
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