Journal
FEMS MICROBIOLOGY LETTERS
Volume 223, Issue 1, Pages 47-51Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1097(03)00329-X
Keywords
anthracnose; iturin A; Bacillus subtilis; Gloeosporium gloeosporioides
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The Bacillus subtilis strain KS03 was isolated, and identified as a biological control agent that inhibits the anthracnose disease fungus Gloeosporium gloeosporioides. The antifungal compound was purified from its culture broth through butanol extraction, diethylaminoethyl (DEAE) Sepharose CL-6B chromatography, and preparative thin layer chromatography. Tandem mass spectrometric analyses (MS/MS), with matrix-assisted laser desorption ionization (MALDI) time-of-fight/time-of-flight (TOF/TOF) mass spectrometry, showed that the antifungal compound was iturin A, a cyclic lipopeptide antibiotic. The major compound, with a molecular mass of 1042 Da, was identified as iturin A(2). (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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