Journal
FEMS MICROBIOLOGY LETTERS
Volume 223, Issue 1, Pages 15-20Publisher
OXFORD UNIV PRESS
DOI: 10.1016/S0378-1097(03)00248-9
Keywords
soil environment; DNA isolation; nycodenz gradient; bacterial artificial chromosome cloning; Escherichia coli-streptomycete artificial; chromosome
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Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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