Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 12, Pages 7075-7080Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0832308100
Keywords
-
Categories
Funding
- NCI NIH HHS [R56 CA096823, R01 CA096823, BC991016, PC010342, CA96823-01] Funding Source: Medline
- NIA NIH HHS [AG08487, R01 AG008487] Funding Source: Medline
- NIBIB NIH HHS [9-P41-EB0011976-16] Funding Source: Medline
- PHS HHS [R33-645644] Funding Source: Medline
Ask authors/readers for more resources
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, in-doleamines, and collagen provides crucial information for physiology and pathology. The efficient application of multiphoton microscopy to intrinsic imaging requires knowledge of the nonlinear optical properties of specific cell and tissue components. Here we compile and demonstrate applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available