Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 126, Issue 1, Pages 99-109Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-0270(03)00071-2
Keywords
retrograde neuronal tracers; fluoro-gold; wheat germ agglutinin; fast blue; trigeminal ganglion; dental pulp; in situ hybridization; immunohistochemistry; dental injury; nociceptors; neurotrophins; neuropeptides
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Funding
- NIDCR NIH HHS [R01 DE09137] Funding Source: Medline
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The dental pulp is a favorable model for studies of interactions between nociceptive sensory neurons and their peripheral target tissues. In the present study, we retrogradely labeled pulpal afferent neurons with an improved method that permits monitoring of changes in neuronal phenotype in response to controlled tooth injuries. The capacity of retrograde neuronal tracers, to diffuse through dentinal tubules was exploited, thereby avoiding the severe injury to the pulp associated with previous tracer application methods. The strategy was to apply the durable fluorescent tracer, Fluoro-gold (FG), to exposed dentin in the floor of shallow cavities in molars, in order to pre-label pulpal neurons in trigeminal ganglia of young adult Sprague-Dawley rats. A high percentage of pupal afferent neurons were retrogradely labeled by application of FG to exposed dentin and the FG fluorescent signal persisted in most labeled neurons for at least 8 weeks. Following tracer application to dentin, the pulp tissue appeared normal histologically, with the exception that a layer of reactive dentin was deposited at the pulp-dentin border beneath the shallow cavities. Assessment of expression of calcitonin gene-related peptide (CGRP) and brain derived neurotrophic factor (BDNF) indicated that pulpal neurons remained in a quiescent, baseline condition cytochemically following application of tracer to cavities in dentin and upregulation of these markers could be detected in neurons that projected to teeth that received a test injury subsequent to tracer application. Thus, labeling of trigeminal neurons via dentinal tubules provides the basis for a useful model for precisely assessing properties of pulpal afferents in both quiescent and activated states. (C) 2003 Elsevier Science B.V. All rights reserved.
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