Journal
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
Volume 60, Issue 2, Pages 299-306Publisher
ARQUIVO BRASILEIRO MEDICINA VETERINARIA ZOOTECNIA
DOI: 10.1590/S0102-09352008000200004
Keywords
Staphylococcus aureus; genomic DNA; DNA isolation
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This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes ( A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction ( HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 105 cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25 mu l.
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